EFFECTS OF INHALATION ANESTHETICS ON PRODUCTION OF KETONE BODIES IN RAT LIVER SLICES

抄録

Effects of two anesthetics, ether and trichloroethylene, on production of ketone bodies in slices of rats liver were investigated. Rats, fasted for 24 hours, and anesthetized, were autopsied and their tissue slices were prepared from liver, brain and kidney cortex. These slices were shaken as customarily in the Warburg apparatus for 60 minutes, and amount of oxygen uptake, and amount of ketone bodies produced or butyrate consumed in liver were determined. Meanwhile total fat in the original tissues of liver, and total and free carbohydrates in liver and kidney cortex were measured. Results were compared with those of the control, of the epinephrine and of CCl₄ administration group. Conclusions were given as follows:
1. Oxygen uptake in brain cortex is lowered by ether and trichloroethylene anesthesia, as by CCl₄ administration.
2. While both free and total carbohydrates of liver decrease in case of ether and trichloroethylene anesthesia, as in case of CCl₄ administration, those in kidney cortex are not influenced by the four substances administered.
3. Production of ketone bodies in liver is promoted by epinephrine administration, while it is reduced by ether anesthesia and CCl₄ administration, and is not influenced by trichloroethylene anesthesia.
Thanks are due to Emeritus Professor Y. Sueyoshi of Keio University for his invaluable advice and encouragement. The author is also indebted to Mr. S. Sugayama for analysis of ether, and to Miss Y. Nagashima and Y. Tajima for their collaborations in carrying out this study.