Abstract
1. Some properties of the L-proline oxidizing enzyme system of the cell suspension of a halophilic Pseudomonas strain No. 101 were studied.
2. Accumulation of L-glutamic acid was shown during the oxidation of L-praline.
3. Isotopic experiments and the oxidation test for glutamic γ-semialdehyde showed that this aldehyde might not be the direct intermediate in the oxidation of L-proline by this bacterium.
The author is indebted to Prof. S. Akabori for the helpful criticism and encouragement during the course of this work, to Prof. F. Egami, Nagoya University, for the gift of the bacterial strain, to Miss T. Wad a for the gift of uniformly C14-labeled L-proline, and to Mr, T. Okuda of the Laboratory for Protein Research of Osaka University for the gift of DL-glutamic γ-semialdehyde dimethylacetal.
