The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Studies on the Hormone-sensitive Lipase of Rat Epididymal Adipose Tissue
Hiromichi OKUDAIkuko YANAGIFrank J. SEKSetsuro FUJII
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1970 年 68 巻 2 号 p. 199-203

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Activity of rat epididymal adipose tissue lipase was significantly influenced according to the way of its expression. When the activity was expressed as μEq per g of tissue, the lipase activity of the tissue of starved rats was higher than that of the tissue of fed rats. On the other hand, when expressed as μEq per mg of protein, the lipase activity of starved rats was lower than that of the tissue of fed rats. Though adrenaline increased the release of free fatty acids from adipose tissue slice, the lipase activity was not activated by this hormone. Our cell free system, in which adrenaline stimulated lipolysis, was compared with Rizack's system. Though remarkable activation was observed in our system, there was no significant stimulation of lipolysis by adrenaline in Rizack's system.
Dichloroisoproterenol considerably inhibited the stimulation of lipolysis by adrenaline in our cell free system as well as in the slice system.
These results support our previous conclusion that there is no hormone-sensitive lipase in adipose tissue.

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© The Japanese Biochemical Society
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