A Bacterial Dextranase Releasing Only Isomaltose from Dextrans
1974 年 75 巻 1 号 p. 105-112
詳細
1974 年 75 巻 1 号 p. 105-112
A novel kind of dextranase which is different from the so-far reported dextranases [α-1, 6-glucan 6-glucanohydrolase, EC 3. 2. 1. 11] was purified 44-fold from the culture supernatant of a soil bacterium (Achromobacter sp.). This dextranase liberates isomaltose from dextrans in an exo-lytic fashion. During digestion of dextrans the degree of polymerization of the reaction products remained 2. Paper chromatograms of digests of dextran showed that the only product was isomaltose. A high-molecular residual substance (limit dextrandextrin), resistant to this enzyme but susceptible to Penicillium luteum dextranase (endo-type) was recovered after exhaustive digestion of macromolecular dextran by the enzyme. The dextranase split panose into isomaltose and glucose, and isomaltotriitol into isomaltose and sorbitol.
