The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Studies on Hormone-sensitive Lipolytic Activity
Yasushi SAITONobuo MATSUOKAHiromichi OKUDASetsuro FUJII
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1974 年 76 巻 5 号 p. 1061-1065

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Lipid micelles were prepared from isolated fat cells by disruption in a hypotonic medium. On addition of 10-6M adenosine 3', 5'-monophosphate (cyclic AMP) or dibutyryl cyclic AMP (DBcAMP) the protein kinase [EC 2. 7. 1. 37] activity but not the lipolysis of these lipid micelles increased greatly. On the other hand, the addition of other lipolytic agents, such as theophylline or adrenaline, or of phospholipase C [EC 3. 1. 4. 3] and CaCl2, increased lipolysis but not protein kinase activity. These results suggest that activation of protein kinase did not necessarily occur on stimulation of lipolysis. Incubation of these lipid micelles with adrenaline resulted in marked lipolysis but no increase in protein kinase activity. These results suggest that the lipolytic action of adrenaline in these lipid micelles was not due to increased lipase [EC 3. 1. 1. 3] activity, as reported by Huttunen and Steinberg (1). The extent of activation of hormone-sensitive lipase by cyclic AMP using the system of Huttunen and Steinberg was very low compared with the extent of stimulation of lipolysis in the lipid micelles or fat cells. Furthermore, cyclic AMP was found to activate not only hormone-sensitive lipase but also lipoprotein lipase, steapsin and kidney and liver lipases.
The mode of activation of lipolysis is discussed on the basis of these results.

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