The Action of Trypsin on Synthetic Chromogenic Arginine Substrates

抄録

A new arginine derivative, N-benzyloxycarbonyl-L-phenylalanyl-L-valyl-L-arginine p-nitro-anilide hydrochloride (ZPVAPA. HCl) was synthesized by the condensation of N-benzyloxy-carbonyl-L-phenylalanyl-L-valine and L-arginine-p-nitroanilide dihydrochloride using dicyclo-hexylcarbodiimide as a coupling reagent and 1-hydroxy-benzotriazole as an additive. L-ZPVAPA.HCI was split by trypsin more readily than N^α-benzyloxycarbonyl-L-arginine p-nitroanilide hydrochloride (L-ZAPA. HCl), N^α-benzoyl-L-arginine p-nitroanilide hydrochlo-ride (L-BAPA. HCl), N^α-tosyl-L-arginine p-nitroanilide hydrochloride (L-TAPA. HCI) and N^α-benzoyl-DL-arginine p-nitroanilide hydrochloride (DL-BAPA. HCl) by factors of 100, 400, 600, and 1, 200, respectively. Low concentrations of dimethyl formamide (DMF) enhanced the trypsin-catalyzed hydrolyses of L-ZAPA. HCl and L-TAPA. HCl, contrary to the findings of other authors that DMF has no effect on the tryptic hydrolysis.