抄録
In the presence of excess amounts of various monosaccharides as acceptors, Bacillus subtilis levansucrase synthesized various heterooligosaccharides as a result of transfer of the fructosyl residue from sucrose. The saccharides produced in the presence of D-glucose, D-mannose, and D-xylose were all non-reducing disaccharides and were identified as β-D-fructofuranosyl-α-D-glucopyranoside (sucrose), β-D-fructo-furanosyl-α-D-mannopyranoside (mannosucrose), and β-D-fructofuranosyl-α-D-xylopyranoside (xylsucrose), respectively. The saccharides produced in the presence of D-galactose were also non-reducing, but consisted of di-, tri-, and tetrasaccharides. The di- and trisaccharides were galsucrose and 6F-β-D-fructofuranosylgalsucrose.
The saccharide synthesized in the presence of L-arabinose was a reducing saccharide and was determined to be 4-O-β-D-fructofuranosyl-L-arabinose. In the presence of D-fructose, several reducing levan oligomers, levanbiose, levantiose, levantetraose, etc., were produced, which were not observed in the synthesis of levan from sucrose alone.
