X-Ray Diffraction Photographs of Chicken Gizzard G-Actin•DNase I Complex Crystals Taken with Synchrotron Radiation

Abstract

X-ray diffraction photographs of a chicken gizzard G-actin•DNase I complex crystal have been recorded using the synchrotron radiation beam emitted by the Synchrotron Radiation Source at Daresbury and the Photon Factory at Tsukuba. The resolution limit was extended to 2.4 Å and the exposure time was reduced approximately by a factor of 10, when data recorded at the Photon Factory, were compared with those recorded with a conventional rotating-anode source. Using a newly designed Weissenberg camera equipped with a multi-layer line screen, the diffraction data in a 36 oscillation range were recorded on a single film up to 3.5 Å resolution.