Biomedical Research
Online ISSN : 1880-313X
Print ISSN : 0388-6107
ISSN-L : 0388-6107
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Enhanced intracellular signaling pathway in osteoblasts on ultraviolet lighttreated hydrophilic titanium
Fuminori IWASAKazuyoshi BABATakahiro OGAWA
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2016 Volume 37 Issue 1 Pages 1-11


Ultraviolet (UV) light treatment of titanium immediately prior to use, or photofunctionalization, reactivates the time-dependent degradation of bioactivity of titanium (biological aging of titanium) and increases its osseointegration capacity beyond the inherent maximal level. Although the initial osteoblast attachment is reportedly enhanced on UV-treated titanium surfaces, the detailed mechanism behind the increase in osseointegration is unknown. This study examined the potential modulation of intracellular signaling pathway in osteoblasts on UV-treated titanium surfaces. Rat bone marrow-derived osteoblasts were cultured on 4-week-old, new, and UV-treated titanium surfaces. The new and UV-treated surfaces were superhydrophilic, whereas the 4-week-old surface was hydrophobic. Although the rate of protein adsorption was similarly increased on the new and UV-treated surfaces compared with the 4-week-old surface, the number of attached cells and their spreading behavior were further enhanced on the UV-treated surface. This additional enhancement was associated with the remarkably upregulated expression of paxillin and phospho-paxillin and exclusive upregulation of Rho GTPase family genes. This study provides with the first molecular evidence of the enhanced initial behavior of osteoblasts on UV-treated titanium surfaces. The enhancement was accentuated and distinct from the new titanium surface with similar hydrophilicity, suggesting that surface properties other than the level of hydrophilicity are responsible.

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© 2016 Biomedical Research Press
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