Biomedical Research
Online ISSN : 1880-313X
Print ISSN : 0388-6107
ISSN-L : 0388-6107
Communications
Overexpressed exocyst complex component 3-like 1 spontaneously induces apoptosis
Shunichi MATSUMOTOJunichi OKADAEijiro YAMADATsugumichi SAITOKazuya OKADATakuya WATANABEYasuyo NAKAJIMAAtsushi OZAWAShuichi OKADAMasanobu YAMADA
Author information
JOURNALS FREE ACCESS

2021 Volume 42 Issue 3 Pages 109-113

Details
Abstract

Exocyst complex component 3-like 1 (EXOC3L1), which regulates insulin secretion, is ubiquitously present in heart, lung, liver, spleen, kidney, muscle, cerebellum, pituitary, adrenal grand, and pancreatic islets. Its deduced amino acid sequence has 31% identity and 53% similarity with Sec6, so they are considered isoforms. Since Sec6 suppresses apoptosis via HSP27, we investigated the involvement of EXOC3L1 expression in apoptosis. We found that overexpressed EXOC3L1 in Chinese hamster ovary cells significantly reduced cultured cell numbers. It also significantly increased apoptotic DNA ladder, caspase 3 activity, and cleavage of caspase 3 compared with the control. Thus, although Sec6 reduces apoptosis by increasing HSP27 phosphorylation, overexpressed EXOC3L1 alone can spontaneously induce apoptosis without apoptotic stimulators or inducers.

Information related to the author
© 2021 Biomedical Research Press
Previous article Next article
feedback
Top