CULTURE OF COLONY-STIMULATING FACTOR (CSF)-PRODUCING HUMAN BLADDER CARCINOMA CELL LINE (HTB9) ON ARTIFICIAL CAPILLARIES

抄録

A human bladder carcinoma cell line HTB9, which produces colony-stimulating factor (CSF), was grown on artificial capillaries in a hollow fiber culture unit. The extracapillary fluid was collected every 2 days, and the levels of CSF and other components of the fluid were evaluated. The level of CSF produced by HTB9 cells in the fluid was sufficiently high and production of CSF was detected for as long as 40 days without a cell passage.