Full Papers
REGULATION OE MORPHOLOGY AND PHYSIOLOGY OF EPITHELIAL CELLS BY COLLAGEN FIBRILS
1988 年 9 巻 1 号 p. 33-45
詳細
1988 年 9 巻 1 号 p. 33-45
Interactions between epithelial cells and collagen fibrils were investigated using primary cultures of newborn rat epidermal cells and adult rat hepatic parenchymal cells. Morphology and the rate of DNA synthesis of these cells were regulated by collagen fibrils in a dose-dependent manner. Collagen fibrils at more than 50 μg/cm2 of growth area greatly reduced spreading of the two types of cells. The rate of DNA synthesis of these cells was inversely related to the quantity of the collagen fibrils available to the cell: when cultured on the collagen fibrils at 500 μg/cm2, the rates of synthesis of epidermal and hepatic cells decreased to 42% and 14% of those on the plastic culture dish, respectively. Collagen in a heat-denatured or a native, but non-polymerized form showed no such actions or less effective on the cells, indicating the importance of a fibril formation in the cell to collagen interactions. The transglutaminase activity of epidermal cells and the secretion of albumin by hepatic parenchymal cells increased proportionately to the amounts of collagen fibrils, suggesting that collagen fibrils support to induce and stabilize the expressions of differentiated functions of the epithelial cells.
