電子位相顕微鏡法による細胞内タンパク質・超分子の可視化

抄録

Electron cryotomography was introduced as the most promising method to visualize proteins and supramolecules inside cells in their intact form. Current spatial resolution with the method is about 5 nm and supramolecules like ribosomes or proteasomes were identified. To extend the resolution to 1 nm for the identification of proteins, electron-phase microscopy could be introduced, by which several supramolecules had been identified for unstained ice-embedded cyanobacteria.