2012 年 52 巻 1 号 p. 014-017
F1-ATPase (F1) is a rotary motor protein which couples ATP hydrolysis/synthesis to rotary motion in a reversible fashion. Compared to other motor proteins, F1 is unique for its high efficiency and reversibility in converting chemical energy into mechanical work. To elucidate its marvelous mechanism, we developed the novel single-molecule manipulation technique with magnetic tweezers and determined the timing of Pi release, which was the last unknown piece of the puzzle of chemomechanical coupling scheme of F1. The established fundamental chemomechanical coupling scheme gave us a clue for high reversibility between catalysis and mechanical work.