Biological Imaging Based on Stimulated Raman Scattering

Abstract

Stimulated Raman scattering (SRS) microscopy allows real-time biomedical imaging based on vibrational contrast, leading to various emerging applications to label-free imaging and Raman-labeled imaging. This review explains the principle, features, and applications of SRS microscopy, and introduces our recent development of SRS spectral microscopy for multicolor, real-time, stain-free tissue imaging.