生物物理
Online ISSN : 1347-4219
Print ISSN : 0582-4052
ISSN-L : 0582-4052
総説
放射光X線をプローブとする多量体タンパク質の機能的分子内運動・1分子解析
関口 博史一柳 光平八木 直人佐々木 裕次
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2015 年 55 巻 4 号 p. 192-195

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Diffracted X-ray Tracking (DXT) is one of single molecule techniques for investigating intra-molecule dynamics of functional proteins. In DXT, a nanocrystal is immobilized on a target protein and the trajectory of its diffracted spot is analyzed as the motion of the protein. We advanced the time resolution of this method and refined the analysis procedures in the last 5 years, and the method has been useful especially for cooperative motion analysis in multimeric proteins. In this article, we review the characteristic features and recent progress of DXT.

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© 2015 by THE BIOPHYSICAL SOCIETY OF JAPAN
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