脂質平面膜法によるイオンチャネルの再構成

融合法の実際と電気生理学的構造解析

抄録

In recent years various ionic channels have been reconstituted in planar lipid bilayers by which rigorous analysis of the electrical behavior of single ionic channels can be made. I have reviewed, in the first half of this article, practical techniques for forming planar bilayers and for incorporating channels into them. The second half deals with a structural analysis of the ion conducting pathway of the reconstituted K⁺ channel from sarcoplasmic reticulum solely by measuring its electrical properties. The electrical analysis of channel structure should be a promissing approach to bridge the gap between the electrical property and the molecular structure of channel proteins in lipid bilayers.