Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Increased Expression of Hsp70 for Resistance to Deuterium Oxide in a Yeast Mutant Cell Line
Keiko UnnoTakahiro KishidoMizue MoriokaShoji OkadaNaoto Oku
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2003 年 26 巻 6 号 p. 799-802

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Labeling with stable isotopes, typically deuterium (D), is powerful tool for studying the functional structure of biomolecules by NMR. Biosynthesis of certain deuterated proteins in microorganisms cultured in deuterium oxide (D2O) is an attractive strategy. However, the growth of almost all microorganisms is inhibited at high concentrations of D2O. We isolated a mutant of yeast that grows well in D2O. The expression of Hsp70 was enhanced in the mutant. The increased expression also endowed the yeast with cold-resistance. The mutant might be useful for biosynthesis of D-labeled biomolecules.

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© 2003 The Pharmaceutical Society of Japan
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