Melanogenesis Stimulation in Murine B16 Melanoma Cells by Piper nigrum Leaf Extract and Its Lignan Constituents

Hideaki Matsuda; Yoshiko Kawaguchi; Miho Yamazaki; Noriko Hirata; Shunsuke Naruto; Yusuke Asanuma; Takayuki Kaihatsu; Michinori Kubo

doi:10.1248/bpb.27.1611

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Melanogenesis Stimulation in Murine B16 Melanoma Cells by Piper nigrum Leaf Extract and Its Lignan Constituents

Hideaki Matsuda, Yoshiko Kawaguchi, Miho Yamazaki, Noriko Hirata, Shunsuke Naruto, Yusuke Asanuma, Takayuki Kaihatsu, Michinori Kubo

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Keywords: Piper nigrum, cubebin, 3,4-dimethoxy-3,4-desmethylenedioxycubebin, 3-desmethoxycubebinin, murine B16 melanoma cell, stimulatory effect on melanogenesis

JOURNAL FREE ACCESS

2004 Volume 27 Issue 10 Pages 1611-1616

DOI https://doi.org/10.1248/bpb.27.1611

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Abstract

A methanolic extract from the leaves of Piper nigrum L. showed a significant stimulatory effect on melanogenesis in cultured murine B16 melanoma cells. Activity-guided fractionation of the methanolic extract led to the isolation of two known lignans, (−)-cubebin (1) and (−)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2), together with a new lignan, (−)-3-desmethoxycubebinin (3). Among these lignans, 1 and 2 showed a significant stimulatory activity of melanogenesis without any significant effects on cell proliferation.

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