Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
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High-Performance Liquid Chromatographic Assay of Clonazepam in Human Plasma Using a Non-porous Silica Column
Mitsuhiro NakamuraKana FukawaTadashi SugiyamaYoshihiro Katagiri
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2004 Volume 27 Issue 6 Pages 893-895

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Abstract

A new HPLC method has been developed for measuring clonazepam (CZP) in plasma, using a reversed-phase non-porous silica column packed with 2 μm particles. CZP in plasma was first purified with a column extraction technique and injected onto a non-porous silica column. The calibration curve was linear from 5—200 ng/ml. The recoveries of CZP added to plasma were more than 94.0%, with a coefficient of variation in the range of 5.1—13.8%. We developed a rapid routine method using a non-porous silica column that was accurate and improved solvent consumption in the measurement of CZP.

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© 2004 The Pharmaceutical Society of Japan
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