Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Essential Oil Composition, Antibacterial and Antioxidant Activity of the Oil and Various Extracts of Ziziphora clinopodioides subsp. rigida (BOISS.) RECH. f. from Iran
Peyman SalehiAli SonboliFereshteh EftekharSamad Nejad-EbrahimiMorteza Yousefzadi
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2005 Volume 28 Issue 10 Pages 1892-1896


The chemical composition of the essential oil obtained from the aerial parts of Z. clinopodioides subsp. rigida (BOISS.) RECH. f. was analysed by GC and GC-MS. Thirty-one constituents accounting to 99.5% of the total oil were identified. Oxygenated monoterpenes (93.3%) were the predominant portion of the oil with pulegone (45.8%), piperitenone (17.4%), p-menth-3-en-8-ol (12.5%) and thymol (8.0%) as the main constituents. Antibacterial activity of the oil and its two main compounds and various extracts of plant were tested against seven Gram-(+) or Gram-(−) bacteria. It was found that the oil and MeOH extract (M) exhibited interesting antibacterial activity. The samples were also subjected to screening for their possible antioxidant activity by using 2,2-diphenyl-1-picrazylhydrazyl (DPPH) assay. The free radical scavenging activity of MeOH extract (M) was superior to all other extracts (IC50=30.7 μg/ml), while the oil was less effective.

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© 2005 The Pharmaceutical Society of Japan
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