Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Regular Articles
Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)
Yuichi SakanoMotoh MutsugaRie TanakaHiroyuki SuganumaTakahiro InakumaMasatake ToyodaYukihiro GodaMasaaki ShibuyaYutaka Ebizuka
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2005 Volume 28 Issue 2 Pages 299-304

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Abstract

Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300 μg/ml). Examination of activity variation among eight taro cultivars indicated that “Aichi-wase” and “Yatsugashira” had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of “Aichi-wase” were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. Further purification of these fractions by preparative HPLC gave three monogalactosyldiacylglycerols and five digalactosyldiacylglycerols as active compounds that showed 28 to 67% inhibitory activities at the concentration 300 μg/ml.

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© 2005 The Pharmaceutical Society of Japan
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