Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Effect of 2-Methoxyestradiol, Buthionine Sulfoximine and Hydrogen Peroxide on the Viability of Renal Carcinoma Cell Lines (ACHN and ACVB)
Yuka HiraiNoriko KawabeYoshiko TsudaSatoru MiyamotoSeigo Iwakawa
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2006 Volume 29 Issue 5 Pages 1064-1067

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Abstract

2-Methoxyestradiol (2-ME), an endogenous metabolite of 17β-estradiol, induces the intracellular accumulation of superoxide anion (O2·−) and buthionine sulfoximine (BSO) is an inhibitor of glutathione (GSH) synthesis. We have examined the combination anticancer effect of 2-ME and BSO accompanied with hydrogen peroxide (H2O2). 2-ME inhibited cell growth in renal carcinoma cell lines (ACHN and ACVB) accompanied by an increase in the intracellular contents of GSH. The combination of 2-ME, BSO and H2O2 showed a significant antiproliferation effect in both ACHN and ACVB. The intracellular levels of reactive oxygen species (ROS) with a combination with 2-ME and H2O2 in ACHN and ACVB pretreated with BSO were markedly increased, which may have contributed to the potential antiproliferative action.

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© 2006 The Pharmaceutical Society of Japan
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