Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Regular Articles
Surface Plasmon Resonance Analysis for the Screening of Anti-prion Compounds
Satoshi KawatakeYuki NishimuraSuehiro SakaguchiToru IwakiKatsumi Doh-ura
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JOURNALS FREE ACCESS

2006 Volume 29 Issue 5 Pages 927-932

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Abstract

The interaction of anti-prion compounds and amyloid binding dyes with a carboxy-terminal domain of prion protein (PrP121—231) was examined using surface plasmon resonance (SPR) and compared with inhibition activities of abnormal PrP formation in scrapie-infected cells. Most examined compounds had affinities for PrP121—231: antimalarials had low affinities, whereas Congo red, phthalocyanine and thioflavin S had high affinities. The SPR binding response correlated with the inhibition activity of abnormal PrP formation. Several drugs were screened using SPR to verify the findings: propranolol was identified as a new anti-prion compound. This fact indicates that drug screenings by this assay are useful.

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© 2006 The Pharmaceutical Society of Japan
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