Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
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Protective Effects of Korean Mistletoe Lectin on Radical-Induced Oxidative Stress
Boh Kyung KimMi Jin ChoiKun Young ParkEun Ju Cho
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2010 Volume 33 Issue 7 Pages 1152-1158

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Abstract

The radical scavenging effects and protective activities against oxidative stress of Korean mistletoe (Viscum album coloratum) lectin were investigated in vitro and with a cellular system using LLC-PK1 renal epithelial cells. The Korean mistletoe lectin (KML) showed 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity with an IC50 value of 42.6 μg/ml. It also exerted nitric oxide (NO), superoxide anion (O2), and hydroxyl radical scavenging activities in concentration-dependent manners. These results suggest that KML is a promising antioxidant by scavenging free radicals. Furthermore, under the LLC-PK1 cellular model, the cells showed declines in viability and increases in lipid peroxidation through oxidative stress induced by sodium nitroprusside (SNP) and pyrogallol, generators of NO and O2, respectively. However, KML significantly and dose-dependently inhibited cell cytotoxicity and lipid peroxidation. In addition, 3-morpholinosydnonimnie (SIN-1), a generator of peroxynitrite (ONOO) formed by simultaneously releases of NO and O2, caused cytotoxicity, lipid peroxidation, and NO overproduction in the LLC-PK1 cells while KML ameliorated ONOO-induced oxidative damage. Furthermore, overexpressions of cyclooxygenase-2 and inducible NO synthase induced by SIN-1 were observed, but KML down-regulated the expression levels of both genes. KML also reduced SIN-1-induced nuclear factor kappa B expression and the phosphorylation of inhibitor kappa B alpha in LLC-PK1 cells. These results indicate that KML has protective activities against oxidative damage induced by free radicals.

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© 2010 The Pharmaceutical Society of Japan
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