Identification and Detection Method for Genetically Modified Papaya Resistant to Papaya Ringspot Virus YK Strain

Kosuke Nakamura; Hiroshi Akiyama; Kiyomi Ohmori; Yuki Takahashi; Reona Takabatake; Kazumi Kitta; Hiroyuki Nakazawa; Kazunari Kondo; Reiko Teshima

doi:10.1248/bpb.34.1648

Communication to the Editor

Identification and Detection Method for Genetically Modified Papaya Resistant to Papaya Ringspot Virus YK Strain

Kosuke Nakamura, Hiroshi Akiyama, Kiyomi Ohmori, Yuki Takahashi, Reona Takabatake, Kazumi Kitta, Hiroyuki Nakazawa, Kazunari Kondo, Reiko Teshima

著者情報

Kosuke Nakamura
National Institute of Health Sciences
Hiroshi Akiyama
National Institute of Health Sciences
Kiyomi Ohmori
Chemistry Division, Kanagawa Prefectural Institute of Public Health
Yuki Takahashi
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University
Reona Takabatake
National Food Research Institute, National Agriculture and Food Research Organization
Kazumi Kitta
National Food Research Institute, National Agriculture and Food Research Organization
Hiroyuki Nakazawa
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University
Kazunari Kondo
National Institute of Health Sciences
Reiko Teshima
National Institute of Health Sciences

キーワード: genetically modified organism, papaya, polymerase chain reaction, genomic DNA

ジャーナルフリー

2011 年 34 巻 10 号 p. 1648-1651

DOI https://doi.org/10.1248/bpb.34.1648

詳細

抄録

Unauthorized genetically modified (GM) papaya (Carica papaya LINNAEUS) was detected in a commercially processed product, which included papaya as a major ingredient, in Japan. We identified the transgenic vector construct generated based on resistance to infection with the papaya ringspot virus (PRSV) YK strain. A specific detection method to qualitatively monitor papaya products for contamination with the GM papaya was developed using the real-time polymerase chain reaction.

責任著者(Corresponding author)

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