Notes
RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method
2014 年 37 巻 8 号 p. 1411-1415
詳細
2014 年 37 巻 8 号 p. 1411-1415
The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2′-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.
