Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits
Yoshiro SaitoHirofumi MisuHiroaki TakayamaShin-ichiro TakashimaSoichiro UsuiMasayuki TakamuraShuichi KanekoToshinari TakamuraNoriko Noguchi
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Supplementary material

2018 Volume 41 Issue 5 Pages 828-832

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Abstract

Selenoprotein P (SeP) is a selenium (Se)-rich extracellular protein. SeP is identified as a hepatokine, causing insulin resistance in type 2 diabetes. Thus, the measurement of SeP in serum has received much attention, and several enzyme-linked immunosorbent assay (ELISA) kits for SeP determination are now commercially available. In the present study, we determined the serum SeP levels by our original ELISA and sol particle homogeneous immunoassay (SPIA) methods and also by commercially available kits, and these determinants were compared. We found a kit-dependent correlation of the determinants with our methods. These results suggest that the selection of kit is critical for comparison with our previous reports and for discussing the relationship between the serum SeP levels and disease condition.

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© 2018 The Pharmaceutical Society of Japan
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