Biological and Pharmaceutical Bulletin
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A Photo-Activatable Peptide Mimicking Functions of Apolipoprotein A-I
Haruka KawaharaNaoki MiyashitaKoki TachibanaYusuke TsudaKyohei MorimotoKohei TsujiAkira ShigenagaAkira OtakaTatsuhiro IshidaKeiichiro Okuhira
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2019 年 42 巻 6 号 p. 1019-1024

詳細
Abstract

Apolipoprotein A-I (apoA-I) plays a critical role in high-density lipoprotein (HDL) biogenesis, function and structural dynamics. Peptides that mimic apoA-I have a short amphipathic α-helical structure that can functionally recapitulate many of the same biologic properties of full-length apoA-I in HDL. Hence, they might be expected to have clinical applications in the reduction of atherosclerosis. However, nonspecific cellular efflux of cholesterol induced by apoA-I mimetic peptides might cause side effects that are, as yet, unidentified. In this study, we developed a photo-activatable peptide, 2F*, which is an 18 amino acid peptide mimicking apoA-I bearing an internal photocleavable caging group that is designed to assume an α-helical structure in response to a light stimulus and trigger efflux of cholesterol from cells. Without light irradiation, 2F* peptide showed a low tendency for the formation of α-helices, and therefore did not associate with lipids and failed to induce efflux of cholesterol. In addition, 2F* did not cause hemolysis under our experimental condition. Mass spectrometry indicated that, after light exposure, the caging group detached from 2F* and it assumed the α-helical structure in the presence of lipids, and enhanced cholesterol efflux from cells. Photo-activatable peptides such as 2F* that control cholesterol efflux following light stimulus may be useful for future atherosclerosis-reducing therapies.

INTRODUCTION

Apolipoprotein A-I (apoA-I) is produced in the liver or small intestine and is secreted in blood as a major component of high density lipoproteins (HDL). A number of epidemiological studies have shown that high levels of HDL cholesterol are associated with a reduced risk of cardiovascular disease.1,2) The key atheroprotective function of HDL is the ability to remove excess cholesterol from cells in the arterial wall and to transport them back to the liver. In addition, HDL also displays other beneficial effects including anti-oxidant, anti-apoptotic, anti-inflammatory and anti-thrombotic properties that have the potential to act at multiple stages throughout the development of atherosclerosis.36) Recombinant apoA-I proteins, in combination with phospholipids, mimic the properties of HDL. Hence, they are being developed as therapeutic agents and have achieved positive results in preclinical studies.7,8) Nevertheless, the clinical use of apoA-I protein is complicated by the need for high doses, leading to high costs. Hence, a peptide-based approach, less costly to produce than full-length apoA-I, is an emerging area of HDL therapy.9,10)

Peptides that mimic apoA-I usually possess an amphipathic helix designated class A, which is critical to the formation of the lipid–protein complex. This is a common structural motif of exchangeable apolipoproteins including apoA-I.11) The amphipathic helix is also necessary for inducing the efflux of cellular cholesterol as nascent HDL particles via in an ATP-binding cassette transporter A1 (ABCA1). Cholesterol-loaded macrophages in the arterial intima release cholesterol primarily through ABCA1,12) thus apoA-I and its mimetic peptides play a critical function in reducing the cholesterol burden of intimal macrophages and promoting the regression of atherosclerosis. However, helical peptides sometimes induce hemolysis of red blood cells by inducing nonspecific cellular cholesterol efflux caused by its highly efficient solubilization of membrane lipids.13,14) Accordingly, a stimulus-inducible peptide mimicking apoA-I that is capable of being activated at atherosclerotic lesions, would be a valuable addition to current HDL therapy.

In this study, we designed and synthesized a novel peptide mimicking apoA-I having a photo-reactive 4,5-dimethoxy-2-nitrobenzyl (DMNB) caging group that inhibits α-helical formation of the synthesized peptide. This novel peptide releases the DMNB group by light-irradiation, allowing α-helical structure formation and the inductions of cholesterol efflux from cells.

MATERIALS AND METHODS

Materials

Dichloromethane, Dimethylformamide, Piperidine, Trifluoroacetic acid (TFA), Acetonitrile (MeCN), Diethyl ether, m-cresol, Thioanisole, 1,2,3-benzotriazol-1-ol monohydrate and 1,2-ethanedithiol were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). N,N′-Diisopropylcarbodiimide was purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). All 9-fluorenylmethyloxycarbonyl (Fmoc) protected amino acids were purchased from CSBio, Inc. (Menlo Park, CA, U.S.A.). CLEAR-amide resin was purchased from Peptide Institute, Inc. (Osaka, Japan). 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was purchased from Sigma-Aldrich (Merck Millipore, Burlington, MA, U.S.A.).

Peptide Synthesis and Light Activation

Peptide 2F (Ac-DWLKAFYDKVAEKLKEAF-NH2) and 2F* (Ac-DWLKAFYDKVA(DMNB)EKLKEAF-NH2) were synthesized on CLEAR-Amide resin by a standard Fmoc solid-phase procedure. As building blocks, a Fmoc amino acid or dipeptide alanine-glutamic acid (Ala-Glu) bearing a 4,5-dimethoxy-2-nitrobenzyl caging group (Ala-(DMNB)Glu), prepared according to the literature,15) was employed. The peptide was purified to greater than 99% purity by reverse-phase HPLC, as assessed by electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS) (2F* MS data in Fig. S1). For analytical HPLC, reaction proceedings were monitored with reverse phased HPLC with C-18 column (Nacalai Tesque, Cosmosil 5C18-AR-II, 4.6 × 250 mm) by a linear gradient of 5–60% MeCN in 0.1% TFA buffer over 30 min at a flow rate of 1.0 mL/min, and monitored by UV detection at 220 nm (HITACHI Pump L-2130 with UV Detector L-2400). For preparative HPLC, the product was purified by reverse phased HPLC with C-18 column (Nacalai Tesque, Cosmosil 5C18-AR-II, 20 × 250 mm) by a linear gradient of 35–50% MeCN for 2F or 30–45% MeCN for 2F* in 0.1% TFA buffer over 30 min at a flow rate of 10.0 mL/min, and monitored by UV detection at 220 nm. The peptides were solubilized in ultrapure water, then diluted to the desired concentration for experimental use. Photolysis was performed at room temperature using a Moritex MUV-202U with the filtered output (> 365 nm) of a 3000 mW/cm2 Hg-Xe lamp. The photo-uncaging reaction of 2F* was monitored by analytical HPLC (Fig. 1B).

Fig. 1. Characterization of Photo-Triggered Cleavage of Peptide 2F*

(A) Photo-removal of the DMNB caging group from peptide 2F*. (B) HPLC profiles of the reaction before (black arrows) and after uncaging (white arrows). The amount of peptide after UV irradiation for 5 min or 30 min were monitored by HPLC and the retention time region in which peaks were detected is enlarged.

Solubilization of 1,2-DMPC Vesicles by Peptides

DMPC vesicles were formed as described previously.16) Briefly, weighed amounts of lipids were dissolved in chloroform–methanol (2 : 1 v/v). The organic solvent was removed by rotoevaporation and the lipid film was hydrated with phosphate buffered saline (PBS) above its melting temperature of 24°C. Large unilamellar vesicles (LUV) were prepared by extruding a lipid suspension through a 200 nm polycarbonate filter yielding vesicles with the same approximate diameter. DMPC concentrations were determined with the choline oxidase-DAOS method (phospholipids C-test Wako kit). Peptides were incubated at 25°C for 30 min with DMPC vesicles (0.2 mg/mL) in PBS at a final peptide concentration of 50 µg/mL with continuous mixing and monitoring the absorbance at 325 nm in a Hitachi spectrophotometer U-3900H.17) Change in turbidity of samples was expressed as fold-change relative to time 0.

Circular Dichroism (CD) Spectroscopy

Far-UV CD spectra were recorded from 200 to 260 nm at 25°C using a Jasco J-1500 spectropolarimeter. The peptide solutions (50 µg/mL) in PBS or the peptides associated with egg phosphatidylcholine liposomes (egg PC; 200 µg/mL) were analyzed by circular dichroism spectroscopy. The α-helical content was determined from the molar ellipticity at 222 nm ([θ]222) using the equation: % α-helix = [(−[θ]222 + 3000)/(36000 + 3000)] × 100.17,18)

Cholesterol Efflux

Cholesterol efflux assay was performed as described previously.17,19) Briefly, Baby Hamster Kidney (BHK) stably transfected cells expressing a mifepristone-inducible human ABCA1 cDNA17,20) were incubated with apoA-I in Dulbecco’s modified Eagle’s medium (DMEM) or HBSS containing 0.02% bovine serum albumin (BSA) for 6 h. The cholesterol content in the supernatant was determined using enzymatic assay after lipid extraction from the supernatant.

Hemolysis Assay

Peptide solutions with different concentrations were mixed with 2% rat blood in PBS and then incubated for 24 h at 37°C. The samples were centrifuged at 750 × g for 5 min, and the hemolysis was assessed as a function of hemoglobin leakage by measuring the absorbance of supernatant at 540 nm. The percentage of hemolysis was determined thus:

  

To obtain 0 or 100% hemolysis, respectively, bloods were incubated either in PBS only or were mixed with distilled water.

Statistics

Statistical analysis was performed using one-way ANOVA followed by the Bonferroni test. Results were regarded as significant for p < 0.05.

RESULTS

For the synthesis of the photoresponsive peptide called 2F*, the photocleavable 4,5-dimethoxy-2-nitrobenzyl (DMNB) group was inserted at the glutamic acid in the peptide 2F. The schematic for synthesized peptide derived by UV-radiation is illustrated in Fig. 1A. The photoreactivity of the peptide 2F* was confirmed as follows. The peptide was irradiated with UV light (> 365 nm) for 5 min or 30 min. The reaction progress was monitored by HPLC (Fig. 1B), and the ESI-MS analysis confirmed that the resulting peptides had the exact same molecular mass as 2F. UV irradiation for 30 min caused almost complete cleavage of the DMNB group from 2F*, resulting in the generation of peptide 2F.

The ability of peptides derived from 2F* to solubilize lipids following UV irradiation was studied by adding peptides to DMPC vesicles, and following the decrease in turbidity as the peptides reorganize the vesicles into smaller structures17) (Fig. 2A). Within 10 min, the original 2F peptide decreased the relative turbidity, confirming that 2F could solubilize DMPC vesicles, consistent with a previous study.21) The activity of the 2F peptide was not influenced by UV-irradiation (Fig. 2A). In the absence of UV-irradiation, peptide 2F* did not induce any lipid solubilization, indicating that the presence of the DMNB group in the peptide successfully inhibited the association of the peptide with lipid. The UV-irradiation decreased the relative turbidity of DMPC vesicles by 80% within 10 min. To determine the secondary structure of the derived peptides, far-UV circular dichroism spectroscopy was utilized (Fig. 2B). The peptide 2F, in the presence of lipids, shows two negative bands at 222 nm and 208 nm in the CD spectrum, typical of an α-helix. Judging by the mean residue ellipticity at 222 nm, the original peptide 2F underwent a large increase in helicity from 7% in the lipid-free state to 63% helicity after lipid reconstitution (Table 1), similar to a previous report.22) The UV-irradiation did not affect the CD spectra of the 2F peptide, indicating that the irradiation did not cause modification or disruption of peptide structures. In the absence of UV-irradiation, peptide 2F* showed a low degree of helicity (18.1%) in the presence of DMPC vesicles. The UV-irradiation of 2F* peptides caused a significant increase in helicity (50.1%). Taken together, these results indicate that UV-irradiation triggered formation of peptide 2F from peptide 2F* by enhancing cleavage of the DMNB group. As a consequence, 2F* peptides acquired the ability to solubilize DMPC vesicles by formation of α-helices promoting peptide-lipid association.

Fig. 2. DMPC Clearance Assay and CD Analysis of Peptides

(A) Relative absorbance of DMPC vesicles incubated with the peptides 2F (blue), 2F* (red), UV-irradiated 2F (light blue) and 2F* (orange). (B) far-UV CD spectra of PC-reconstituted peptides with or without UV-irradiation (30 min). (Color figure can be accessed in the online version.).

Table 1. α-Helical Content for 2F and 2F* Peptides, with or without UV Light Exposure in the Lipid-Free or Lipid-Bound States
PeptidesHelicity (%)
In Tris bufferIn DMPC
2F7.062.6
2F + UV7.163.4
2F*7.018.1
2F* + UV7.150.1

Next, we examined whether 2F* peptides promote cholesterol efflux from cells in the presence of UV-irradiation. The peptide was incubated with BHK cells expressing mifepristone-inducible ABCA1 and the amount of released cholesterol in the supernatant was measured in the presence or absence of UV-irradiation. The original 2F peptide promoted cholesterol efflux from the cells in a dose-dependent manner (Fig. 3A). In the presence of mifepristone, the cellular cholesterol efflux was further increased, indicating that the peptide promoted ABCA1-dependent cholesterol efflux. Without UV-irradiation, regardless of the presence or absence of mifepristone, the peptide 2F* did not induce cholesterol efflux over a range of concentrations (10–50 µg/mL) (Fig. 3A). Following UV-irradiation, the 2F* peptide promoted the cholesterol efflux to a comparable level with the original 2F peptide in the presence of mifepristone (Fig. 3B). These results indicate that UV-irradiation of 2F* peptides mediated specific cholesterol efflux in an ABCA1-depenent manner.

Fig. 3. Cholesterol Efflux by Peptides with or without UV Irradiation from Cells Expressing or not Expressing ABCA1

(A) BHK/ABCA1 cells were treated with (black bars) or without (white bars) mifepristone to upregulate ABCA1 expression. 2F or 2F* peptides without UV-irradiation (10, 25, 50 µg/mL) were then incubated with the mifepristone-treated or untreated BHK/ABCA1 cells for 6 h at 37°C. The amounts of cholesterol in the media were measured as described in Materials and Methods. (B) 2F (10 µg/mL), 2F* (10 µg/mL), 2F or 2F* with UV irradiation for 30 min were incubated with the mifepristone-treated or untreated BHK/ABCA1 cells for 6 h at 37°C. Results are expressed as the mean of triplicates±standard deviation( S.D.).

Toxicity analysis of 2F peptides showed that they increased red blood cell hemolysis in a dose-depending manner. Hemolysis caused by newly synthetized 2F* peptides showed that hemolysis was increased by UV irradiation in a dose dependent manner. Almost 20% of red blood cells were lysed at 100 µg/mL (Fig. 4) in 24 h at a temperature of 37°C, compared to a low hemolytic activity (less than 5%) in the absence of UV-irradiation (Fig. 4).

Fig. 4. Hemolysis of Red Blood Cells by Peptides

Peptides were incubated with red blood cells for 24 h at 37°C followed by centrifugation. The supernatant was measured at an optical density of 540 nm. Results are expressed as the mean of triplicates S.D. (#) indicates significant differences (#p < 0.05, ##p < 0.01) at 100 µg/mL.

DISCUSSION

The most important structural protein in HDL, ApoA-I, consists of 243 amino acids with 10 amphipathic α-helices that are crucial for its efficient interaction with lipids. Recently, there has been increasing interest in the application of peptides that mimic the amphipathic helices in apoA-I as therapeutic agents.9,10) In this study, we developed a novel photo-activatable peptide mimicking apoA-I, programmed to induce lipid solubilization by UV-irradiation triggered by α-helix formation. Peptide 2F* was designed to contain a photo-cleavable caged glutamate that inhibits the hydrogen bonding required for the formation of α-helices (Fig. 1A). Peptide 2F* showed a low helix content in the presence of lipid vesicles (Fig. 2B, Table 1), no lipid solubilizing activity (Fig. 2A), very little hemolysis activity (Fig. 4), and no cholesterol efflux activity from cells (Figs. 3A, 3B). Of significance, following UV-irradiation, peptide 2F* showed a high helical content (Fig. 2B), significant lipid solubilizing activity (Fig. 2A), high hemolytic activity (Fig. 4) and cholesterol efflux activity (Fig. 3B), all consistent with the original 2F peptide. These results confirm that the 2F* peptide acquired the original activity of 2F peptide by liberating the DMNB group photolytically via UV-irradiation.

A series of apoA-I-mimetic peptides was designed to recapitulate the various in vivo beneficial function of HDL including reverse cholesterol transport activity, anti-inflammation, anti-oxidation and anti-proliferation,2326) and some of these peptides have already shown a significant level of anti-atherogenicity in preclinical settings.27,28) However, some apoA-I-mimetic peptides have been reported to be hemolytic by nonspecific enhancement of cellular cholesterol efflux from red blood cells due to their high lipid solubilizing activity.13,14) Peptide 2F*, at higher concentrations, caused very little hemolysis unless activated by UV irradiation, while the original 2F peptide caused considerable hemolysis (Fig. 4). This suggests that the 2F* peptide is safe for intravenous administration compared to the original 2F peptide. It is possible that we might control not only cholesterol efflux at sites of atherosclerotic lesions, but systemic peptide-induced hemolysis by light stimulus under physiological conditions, which may provide advantages over the current apoA-I mimic peptides.

ApoA-I or its mimetic peptides induce the efflux of cholesterol and phospholipids from cells in both an ABCA1-dependent and -independent manner. ABCA1-mediated cholesterol efflux begins with the direct interaction of apoA-I or helix-type peptides to the cell membrane surface of ABCA1, and apoA-I or its peptide recruit cellular phospholipid and cholesterol translocated by ABCA1 to assemble HDL particles.29) ABCA1-independent cholesterol efflux involves a non-specific diffusion of lipids from the cell surface, in which cholesterol is trapped by various extracellular acceptors.

Several apoA-I mimetic peptides induce a non-specific cholesterol efflux, which leads hemolysis by associating with the surface of red blood cells and extracting lipids in an ABCA1-independent manner. A considerable hemolytic property has been reported about the peptide of 37 pA which linked two 2F with proline (2F-Pro-2F).13) Additionally systemic administration of apoA-I mimetic peptides may be saturated with lipids provided from plasma lipoproteins or surrounding cells before reaching the target site.13) Therefore, peptides that control the cholesterol efflux with external stimulus may be useful both for minimizing the risk of toxicity and for maximizing the efficacy.

Photoactivation of caged compounds enables the spatial and temporal control of biomolecules in living cells or tissues. Such light-triggered systems, however, still has technical difficulties in in vivo use due to limited light penetration into the body. One strategy to overcome this limitation is to utilize the two-photon absorption process, where photosensitive groups simultaneously absorb two photons of near-infrared (NIR) light that can show relatively high levels of penetration to achieve a photochemical reaction similar to absorbing one photon of UV light.30) Interestingly, upconverting nanoparticles (UCNP) that convert from NIR to UV or visible light by virtue of two-photon absorption process is under investigation; this can trigger the photoreactions of adjacent photosensitive reagents.31) The combination of these materials with photosensitive peptides may lead to the efficient induction of the photochemical process in deep tissues, which expand the utility of our approach for future therapeutic use.

In conclusion, we have developed a new class of apoA-I-mimetic peptides and showed their superior physicochemical properties and biological activities in vitro. Through further studies on 2F* and improvements to prove its effectiveness in animal models, proof-of-concept results will be obtained for a novel approach for treatment of atherosclerosis.

Acknowledgments

The authors are grateful to Dr. Theresa M. Allen for her helpful advice in developing the English manuscript. This study was supported, in part, by a Grant-in-Aid for Scientific Research (25430164, 16K08236 and 16KK0203) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, by the Nagai Foundation, Tokyo, by Takahashi Industrial and Economic Research Foundation, Tokyo, and by a research program for development of intelligent Tokushima artificial exosome (iTEX) from Tokushima University.

Conflict of Interest

The authors declare no conflict of interest.

Supplementary Materials

The online version of this article contains supplementary materials.

REFERENCES
 
© 2019 The Pharmaceutical Society of Japan
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