Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Capillary-Zone Electrophoretic Analyses of the Proteins and Amino Acid Components in Cerebrospinal Fluid of Central Nervous System Diseases
Atsushi HIRAOKAIsao MIURAMunekazu HATTORIItaru TOMINAGASachiko MACHIDA
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1993 Volume 16 Issue 10 Pages 949-952

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Abstract

Analysis systems for protein and amino acid components in cerebrospinal fluid (CSF) from patients with a variety of central nervous system (CNS) diseases were established employing capillary-zone electrophoresis (CZE). Under the conditions employed, the major proteins in CSF and the concurrent sera were separated into deveral respective fractions, and in the obtained electropherograms of serum proteins, the % values of areas of each major protein fraction (from γ-globulin to albumin) were in close accord with those in the densitograms of stained protein bands after electrophoresis on cellulose-acetate strips which had been done as a routine laboratory test in the hospital. Some unusual migration patterns of major proteins were observed in various CNS diseases, and an increase of glutamine in hepatic encephalitis was also detected in the electropherograms. A peak responsible for a minor protein component with the molecular weight of 10000-30000, which was tentatively identified as β-trace protein, was found to be contained in all the CSF samples examined, and its concentration levels were higher in some patients with cerebral infarction and multiple sclerosis. These results suggest that CZE can become a powerful aid in analyses of the protein and amino acid components in CSF for biochemical diagnosis of CNS diseases.

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