Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Studies on Thermophile Products. VI. Activation of Mouse Peritoneal Macrophages by Bis(2-hydroxyethyl) Trisulfide
Yasuhiro KOHAMAKentaro IIDAKeishi TANAKATaro SEMBAMasahiko ITOHTetsuyuki TERAMOTOKazutake TSUJIKAWATsutomu MIMURA
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1993 Volume 16 Issue 10 Pages 973-977


The biological effects of a cytotoxic substance (BS-1), isolated from Bacillus stearothermophilus UK563 and identified as bis(2-hydroxyethyl) trisulfide, on elicited mouse peritoneal macrophages induced by a casein injection, were investigated in vitro. BALB/c mouse macrophages treated or pretreated with BS-1 (1-10 μg/ml) showed cytotoxicity against syngeneic DBA/2 mouse P815 mastocytoma. BS-1 also showed weak cytotoxicity directly against P815 in the absence of macrophages. BS-1 significantly increased the glucose consumption of macrophages without producing cytotoxicity. This trisulfide compound increased nitric oxide formation, interleukin-1 production and prostaglandin E2 release in macrophages. It did not, however, increase the production of active oxygen species in macrophages, but it reduced cytochrome c in the presence of phagocytes. These results indicate that BS-1 activates macrophages to the cytolytic stage.

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