Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Inhibitory Effects of S-(1, 2-Dicarboxyethyl)glutathione on Collagen-Induced Platelet Aggregation; Enhancements of Cyclic AMP Level and Adenylate Cyclase Activity in Platelets by S-(1, 2-Dicarboxyethyl)glutathione
Seiji TSUBOIEmiko FUJIWARAKazumi OGATAAkihiro SAKAUETaiji NAKAYAMAShinji OHMORI
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1993 Volume 16 Issue 11 Pages 1083-1086

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Abstract

S-(1, 2-Dicarboxyethyl)glutathione (DCE-GS) in addition to being present in the liver, lens, and heart, also inhibited platelet aggregation. To clarify these inhibitory effects, the role of DCE-GS in the release of ATP and serotonin from platelets was studied, as was thromboxane A2 formation, cyclic AMP level and adenylate cyclase activity in human platelets. The results are as follows : DCE-GS at a concentration of 1.3 mM inhibited ATP and serotonin release from platelets induced by collagen, by 77.4±4.3 and 78.7±6.3%, respectively. At 1.5 mM DCE-GS also inhibited the formation of thromboxane B2 by 79.6±4.1%. Incubation of human platelet rich plasma with 2 mM of DCE-GS for 10 min increased the cyclic AMP level and the activity of adenylate cyclase by 204±28 and 211±11.7%, respectively. These results suggest that the inhibitory effect of DCE-GS on the platelet aggregation induced by collagen is due to an increase in the cyclic AMP level in plateletds, which in turn may be due to enhancement of the activity of adenylate cyclase.

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