Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Heat Stable Interleukin-1 Activity and Activity Inhibiting Thymocyte Proliferation in Inflammatory Polymorphonuclear Leukocytes
Satoru YUIDe YANGMasatoshi YAMAZAKI
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1993 Volume 16 Issue 11 Pages 1150-1155

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Abstract

The present study demonstrates the co-existence of heat stable interleukin-1 (IL-1) and IL-1-suppressing activity in murine inflammatory polymorphonuclear leukocytes (PMNs). When the IL-1 activity in casein-induced PMN lysate was examined by lymphocyte activating factor assay, it peaked at a concentration of 2.5×106 cells per ml, but this activity was reduced to nothing at higher concentrations, suggesting the co-existence of IL-1 and IL-1-suppressive factors in PMNs. Although IL-1 has been reported to be inactivated by heat treatment, approximately one-fourth of the IL-1 activity was restored after treatment at 100°C for 10 min. In gel chromatography, the IL-1 activity of the PMN-soluble fraction was eluted in two broad peaks with apparent molecular sizes of 17-23 kDa and 23-43 kDa, respectively. Since the heat stable IL-1 activity was mainly eluted in fractions corresponding to the latter peak, and was neutralized by IL-1 receptor antagonist (Il-1ra) or anti-IL-1α antiserum, the heat stable Il-1 is suggested to be precursor form IL-1α. A high concentration of soluble fraction from PMN lysate, in contrast, completely suppressed the activity of recombinant IL-1α or β, and the suppressive activity was heat labile. Since thymocyte mitogenesis by 5 μg/ml of concanavalin A (Con A) with or without IL-2 was also completely suppressed, the suppressive factor(s) may not be IL-1ra or transforming growth factor β which were reported to be present in PMNs.

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