Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Inhibitory Effect of β-Glucans on Zymosan-Mediated Hydrogen Peroxide Production by Murine Peritoneal Macrophages in Vitro
安達 禎之大野 尚仁宿前 利郎
ジャーナル フリー

1993 年 16 巻 5 号 p. 462-467


Effects of the pretreatment of murine peritoneal macrophages with several polysaccharides on the production of H2O2 induced with unopsonized zymosan were examined. Pretreament with most of (1→3)-β-D-glucans for 6 h at 37°C inhibited the zymosan-mediated H2O2 production by macrophages. The phorbol myristate acetate (PMA)-mediated H2O2 production was not affected by the pretreatment. The pretreatment of macrophages with (1→3)-β-D-glucans decreased the ability to ingest unopsonized zymosan, but did not affect the ingestion of IgG-coated sheep red blood cells (IgG-SRBC). These results suggested that the pretreatment with (1→3)-β-D-glucans interfered with the interaction of macrophages to zymosan and that the occupation of the receptor for the (1→3)-β-D-glucans inhibited zymosan-mediated production of H2O2 by macrophages. Chemical modification by substitution with carboxymethyl groups or hydroxyethyl groups of a (1→6)-branched (1→3)-β-D-glucan reduced the inhibitory effect of pretreatment on zymosan-mediated H2O2 production. The above results indicated the possibility that murine peritoneal macrophages possess certain receptors for β-anomeric glucans, and one ligand specificity of the receptors is to restrict the intact (1→3)-β-D-glucosyl back bone.

© The Pharmaceutical Society of Japan
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