Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Autoradioluminography, a Novel Quantitative Method of TLC-Autoradiography
奥山 光伸羽鳥 泰彦重松 昭世
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ジャーナル フリー

1994 年 17 巻 5 号 p. 559-563

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In pharmacokinetic studies, ordinary methods of identifying or quantitatively measuring metabolites have been complicated and become difficult to handle. Here, a novel TLC-autoradioluminography (TLC-ARLG) is reported. This technique is especially suited for quantitatively measuring a minute amount, below-1 ng, of many radioactive metabolites in plasma, tissues or organs, thus eliminating cumbersome extraction procedures. The method has an extremely wide dynamic range and could offer a powerful analytical technique for studying a radioactive compound and its metabolites in a biological specimen. Autoradioluminography is a new quantitative technology developed by combining autoradiography with radioluminography. Radioluminography is a kind of radiography in which a specially designed radio-sensor plate, called an "Imaging plate (IP), "developed by Fuji Photo Film Co., Ltd., is used. The radio-sensor is made of fine crystals of BaF : Eu2+, and the technology is based on photo-luminescence phenomena.

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© The Pharmaceutical Society of Japan
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