Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Preparation of Monoclonal Antibodies for Immunoblotting Human Growth Hormone Receptor and Growth Hormone-Binding Protein
Yoshiro SAITOTakeshi YAMAZAKIKazuhiro SUZUKIHideharu IKEBUCHIAkira ASAKURAYoshimi OTAJunichi SAWADATadao TERAO
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1994 Volume 17 Issue 7 Pages 983-986

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Abstract

Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma.

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© The Pharmaceutical Society of Japan
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