Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Purification and Properties of a Plant β-D-Glucuronidase from Scutellaria Root
Fumio IKEGAMIKaori MATSUNAEMasafumi HISAMITSUTakashi KURIHARATakeshi YAMAMOTOIsamu MURAKOSHI
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1995 Volume 18 Issue 11 Pages 1531-1534

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Abstract

β-D-Glucuronidase (baicalinase, GUS [EC 3.2.1.31]) activity in the crude drug, Scutellaria root, was assayed in line with the quality control standards of Kampo (Japanese herbal) medicines. GUS was purified to homogeneity in the purification steps including DEAE-Sepharose Fast Flow and chromatofocusing used PBETM94 and Polybuffer 74. These results suggest that the Scutellaria GUS is composed of 55 kDa active subunits and that the isoelectric point of this enzyme is pH 5.4. Optimal catalytic activity was found at pH 4.7 in the pH range 3.6-6.2 in 50 mM Na-citrate buffer. The purified enzyme hydrolyzed baicalin and wogonin glucuronide, but did not hydrolyze glycyrrhizin or some β-glucosides found in other crude drugs. GUS activity in several crude drugs is also described.

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© The Pharmaceutical Society of Japan
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