Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Studies on the Activation Mechanisms of Guanylyl Cyclase by Serotonin, Probably through a Novel Subtype of Serotonin Receptor (5-HTGC)
Michihisa TOHDARei IMAIZUMIAkiko SEKIYANaoko ITOHYasuyuki NOMURA
Author information
JOURNALS FREE ACCESS

1995 Volume 18 Issue 8 Pages 1072-1075

Details
Abstract

Characterization of the serotonin-induced increase in guanosine 3', 5'-cyclic monophosphate (cyclic GMP) was investigated and compared with that induced by atrial natriuretic peptide (ANP) in NG108-15 cells. The cyclic GMP formed by serotonin or ANP was transported in a similar manner to the extracellular medium, although the cyclic GMP formed by bradykinin was not. Serotonin and ANP raised cyclic GMP additively. Serotonin-induced cyclic GMP formation was completely inhibited by pretreatment with 100nM 12-o-tetradecanoylphorbol 13-acetate (TPA), although that induced by ANP was only partialy inhibited and the effects were blocked by pretreatment with staurosporin. In membrane preparations, ANP stimulated cyclic GMP formation in the presence of ATP, but serotonin did not. Serotonin-stimulated cyclic GMP formation was found to occur in neuroblastoma N18TG-2, but not in glioma C6Bu-1. These results suggest that a novel subtype of serotonin receptors (5-HTGC) which stimulates membrane-bound guanylyl cyclase, different from that stimulated by natriuretic peptide, may exist especially in neurons.

Information related to the author
© The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top