Urinary Excretion of Thiobarbituric Acid Reactive Substances of Healthy Subjects Supplemented with a High Dose of d-α-Tocopherol

抄録

The level of urinary thiobarbituric acid reactive substances (TBARS) consisting of bound forms of malonaldehyde and, to a lesser extent, other aldehydes is one of the indices of lipid peroxidation status. Levels of urinary TBARS in healthy subjects before and after supplementation with a high dose of d-α-tocopherol were measured using high performance liquid chromatography. Four healthy Japanese were given a supplement of 300mg d-α-tocopherol/d, about 40-fold higher than the normal intake recommended, for a period of 50d. Levels of urinary TBARS (nmol/kg body weight·h) within-day, before and after supplementation with d-α-tocopherol, exhibited similar behavior and levels of daily urinary TBARS (nmol/kg body weight·d) were unchanged by d-α-tocopherol supplementation. These results indicate that supplementation with a high dose of d-α-tocopherol does not affect the level of urinary TBARS.