A New Method for Permeabilization of the Plasma Membrane of Cultured Mammalian Cells. V. A Standard Procedure Recommended for Permeabilization by Vortex-Stirring with High Molecular Weight Polyacrylic Acid

Abstract

As we previously reported, permeabilization of the plasma membrane was effectively achieved by vortex-stirring the mammalian cells in the presence of high molecular weight polyacrylic acid. Based on our present findings, as well as those of our previous studies, optimal conditions for permeabilization were considered in terms of the structure and dosage of a polymer, the constituent of the medium, the conditions of cells, vortex-stirring and post-vortex-stirring, etc. A standard procedure tentatively recommended is described.