Binding Assay of Low Density Lipoprotein to Asp-Hemolysin from Aspergillus fumigatus

Abstract

Binding activity of Asp-hemolysin to low density lipoprotein (LDL) was measured. LDL binds to Asp-hemolysin with an affinity as high as the LDL receptor. The apparent K_d, determined by Scatchard plot analysis, was 8.9×10^-9 M [¹²⁵I]LDL. Asp-hemolysin is thus identified as a novel LDL-binding protein.