Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Influence of Aging on Rolipram-Sensitive Phosphodiesterase Activity and [3H] Rolipram Binding in the Rat Brain
Michihisa TOHDAToshihiko MURAYAMAShinsuke NOGIRIYasuyuki NOMURA
ジャーナル フリー

1996 年 19 巻 2 号 p. 300-302


To clarify the quantitative and qualitative changes in type IV phosphodiesterase (PDE IV) with aging, phosphodiesterase (PDE) activity and [3H] rolipram binding in the cytosolic fraction from the brains of young and aged rats were examined. In all areas of the aged (100-week-old) rat brain except for hippocampus, the PDE activity was decreased by about half that in the young (10-week-old) animals. However, inhibition % by 100μM rolipram and by 100μM isobutylmethylxanthine (IBMX) was not significantly different between the aged and young rats. On the other hand, [3H] rolipram binding and rolipram-sensitive PDE activity did not change with aging in the hippocampus, although both were decreased in other brain regions. These results suggest that PDE IV does not decrease with aging and maintains its cAMP degrading activity in the hippocampus. It may be involved in the dysfunction of the hippocampus with aging.

© The Pharmaceutical Society of Japan
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