1999 Volume 6 Pages 9-12
We previously obtained transgenic rice plants via particle bombardment technology by using the scutellar tissues of mature embryos after a 7-day culture on the callus induction medium. In this experiment, the early events in rice scutellar tissues bombarded with DNA-coating gold particles were observed histologically. After bombardment, the gold particles were uniformly dispersed over the surface of the scutellar epithelium tissue which consisted of the dividing palisade cells. There were some cells containing a single or several particles on the surface of scutellar tissues. Although the particles hardly penetrated into internal cells, the introduction of foreign DNA into only the surface cells of epithelium tissue was sufficient for production of transgenic rice plants, because the single epithelium cell has the competence for transformation and regeneration.