Synthesis of polymer particles with specific binding sites for lysozyme by a molecular imprinting technique and its application to a quartz crystal microbalance sensor

Abstract

Acrylic acid and the acrylamide were radically polymerized with (NH₄)₂S₂O₈ in a phosphate buffer containing lysozyme, surface-modified silica beads, and cross-linkeres. A polymer layer having a recognition site to lysozyme on a silica-gel surface was formed using a molecular imprinting technique. The modified silica gel was able to grasp lysozyme selectively compared with non-modified silica gel. Even after an operation of binding and releasing of lysozyme to the modified silica-gel has been repeated several times, the rebinding ability does not decrease. A ssensor that use the quartz crystal microbalance method, which involves a polymer layer having a recognition site to lysozyme, was made. The quartz crystal was coated with a polymer layer having a recognition site to lysozyme and set to the cell. When a lysozyme aqueous solution was dropped onto the cell, good sensitivity and response were shown. It turns out that it can be used as a sensor film.