2002 Volume 51 Issue 6 Pages 429-435
Lactic acid, uric acid, xanthine and tyrosine in human sweat were determined by HPLC. These chemical substances are commonly used as important clinical makers. A handy method for sweat sampling was devised. After filling 40 μl of 1% ethanol aqueous solution in a 0.6 ml vial, the content was contacted with a finger and collected sweat for 5 min. Concurrently, sweat secretion was estimated by the identical location on the other hand by measuring with a perspiration rate meter. The average values for 8 young subjects were 154 mM, 150 μM, 225 μM and 2.25 mM for lactic acid, uric acid, xanthine and tyrosine, respectively. The variation of lactic acid in sweat after the intake of wine was studied. Although the amount of sweat secretion was almost constant before and after the intake of wine, the lactic acid concentration in sweat showed a maximum at around 25 to 45 min after its intake. We suppose that lactic acid in sweat is induced from blood capillaries.
