分析化学
ノート
温度応答性クロマトグラフィーによるカテキン類の分離
坂本 千賀子岡田 裕司金澤 秀子菊池 明彦岡野 光夫
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52 巻 (2003) 10 号 p. 903-906

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We have developed a new method of chromatography, temperature-responsive chromatography, using packing materials modified with poly(N-isopropylacrylamide) (PNIPAAm). The temperature-responsive polymer, PNIPAAm, shows a reversible and sharp phase transition in an aqueous solution. The PNIPAAm-modified stationary phase alters the properties of the surface from hydrophilic to hydrophobic in response to temperature changes at the phase-transition temperature. In this study, we examined the separation of five catechins (epigallocatechin, epicatechin, catechin, epigallocatechin gallate and epicatechin gallate) using a PNIPAAm-modified column without changing the composition of the mobile phase. In this method, interactions between catechins and the stationary phases were controlled by the temperature. The retention times of all catechins were retarded, because the interaction increased at elevated temperature. We achieved the separation of catechins using only an aqueous solution as a mobile phase by temperature-responsive chromatography.

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© The Japan Society for Analytical Chemistry 2003
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