Detection of tetramethylthiuram disulfide and related compounds using the chemiluminescence of tris(2,2'-bipyridyl)ruthenium(III) cation

Abstract

The chemiluminescence (CL) of tris(2,2'-bipyridyl)ruthenium(III) cation was applied to the determination of tetramethylthiuram disulfide (TMTD). In this work, a sample was injected to an HPLC system with Chromolith Performance RP-18e. The elueted sample and CL reagent were mixed, and sent to a CL-detector. The detection limit of TMTD using the HPLC system was 0.15 pmol (S/N = 3). The calibration curve was linear over the injection amount range from 0.6 to 20 pmol. To improve the sensitivity of the CL method, the water that generated the background was removed from the system. As a result, the sensitivity was improved.