ノート
金基板上でのグアニン塩基の酸化電流に基づくDNA量の測定
2006 年 55 巻 12 号 p. 975-978
詳細
2006 年 55 巻 12 号 p. 975-978
Electrochemical oxidation on a nucleic acid base, such as guanine, adenine, thymine, cytosine, was examined; only guanine could be oxidized on a gold electrode. Because deoxyguanosine, deoxyguanosine monophosphate were also oxidized on the electrode, guanine in DNA would be oxidized on the electrode. To prove DNA oxidation, thiolated-single strand DNA (ssDNA) was immobilized on a gold electrode, and electrochemical measurements were performed: differential pulse voltammetry (DPV) and chronoamperometry were examined. The voltammogram of DPV on an ssDNA-modified electrode had an oxidation peak at +1.17 V vs. Ag/AgCl. The oxidation current was caused by the oxidation of guanine in the DNA-base. The electrical charge difference between a modified electrode and a bare electrode on chronoamperometry agreed with the guanine amount of ssDNA on gold, which was obtained from the result of a quartz crystal microbalance.
