3,4,5,6-テトラフルオロ-2-カルボキシフェニルフルオロンとマンガン（II）を用いるヒストンのメンブランフィルター捕集・定量

臣永 宏; 門林 宏子; 山口 敬子; 神野 伸一郎; 西村 美智子; 藤田 芳一

doi:10.2116/bunsekikagaku.55.127

Note

Determination of Histone by Membrane Filter Collection with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and Manganese(II)

Hiroshi TOMINAGA, Hiroko KADOBAYASHI, Takako YAMAGUCHI, Shinichiro KAMINO, Michiko NISHIMURA, Yoshikazu FUJITA

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Keywords: histone determination, 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone, manganese(II), membrane filter

JOURNAL FREE ACCESS

2006 Volume 55 Issue 2 Pages 127-131

DOI https://doi.org/10.2116/bunsekikagaku.55.127

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Abstract

We reported on a spectrophotometric determination of DNA binding protein, histone, with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese(II) in a previous paper. In the present work, preconcentration method for the determination of histone, which involves the filtration of a histone - 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone - manganese(II) aggregate on a membrane filter, followed by its redissolution or tristimulus colorimetry, is described. Each of these methods is about 10-times more sensitive than the usual spectrophotometry.

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