Abstract
The chemiluminescence (CL) reaction of tris(2,2'-bipyridine)ruthenium(III), [Ru(bipy)3]3+, is induced by a coreactant, such as an aliphatic tertiary amine. Therefore, many analytical applications of [Ru(bipy)3]3+ as a CL reagent have been reported. Finding a novel molecule that reacts as a coreactant led us to develop this CL detection technique for a wider range of compounds. We have found that [Ru(bipy)3]3+ reacts with conjugated diene compounds, or five-membered heteroaromatic compounds to emit chemiluminescence in aqueous solutions. In addition, we confirmed the effect of functional groups beside nitrogen of aliphatic amines on the [Ru(bipy)3]3+ CL reaction. Based on these findings, we proposed new chromatographic methods for the determinations of various compounds in environmental, biological, and food samples.
